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DNA Base Pairs Calculator

Calculate complementary DNA sequences and analyze base pair compositions with our easy-to-use calculator.

How to Use This Calculator

  1. Enter your DNA sequence using A, T, G, and C letters
  2. Click "Calculate" to get the complementary sequence
  3. View the base pair composition analysis
  4. Check the GC content percentage
  5. Review any warnings about invalid nucleotides

Understanding DNA Base Pairs

DNA base pairs are the building blocks of the double helix structure. The four nucleotide bases in DNA are:

  • Adenine (A) - pairs with Thymine
  • Thymine (T) - pairs with Adenine
  • Guanine (G) - pairs with Cytosine
  • Cytosine (C) - pairs with Guanine
Base Pairing Rules

The base pairing in DNA follows specific rules:

  • A-T pairs form two hydrogen bonds
  • G-C pairs form three hydrogen bonds
  • Base pairs must be complementary
  • The DNA strands run antiparallel (5' to 3' in opposite directions)
Importance of GC Content

The GC content of DNA is an important characteristic that affects its properties:

  • Higher stability due to three hydrogen bonds
  • Influences melting temperature
  • Important for PCR primer design
  • Varies between different organisms and genomic regions

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